SealPlate^® Sealing Films for ELISA, EIA, and Similar Assays

SealPlate^® 50 µm thickness polyester sealing films minimize evaporation, prevent spillage and contamination between wells, and provide a secure seal, not just a cover. Secure sealing of all wells eliminates "edge effects" in sensitive ELISA assays. SealPlate sealing films can also be used with tissue-culture plates for short-term storage, incubation, and containment of biohazards. SealPlate sealing films are non-pierceable. Each SealPlate sealing film measures 79.4 x 146.1 mm. ThinSealTMpolyester sealing films are just 25 µm thick, clearer and marginally peirceable with single-channel pipettes for sample recovery. ThinSeal sealing film dimensions are 79.4 x 141.0 mm.

• functional temperature range from -40°C to +120°C
• available sterile and non-sterile

A 140 µm thick hydrophobic porous rayon sealing film including medical-grade adhesive for tissue culture plates, bio-blocks, and 96-well plates where gas exchange is necessary for cell or bacterial growth. AeraSeal sealing films minimize cross-contamination, spillage and evaporation. They allow uniform air and CO2 exchange for all wells, unlike plate lids which favor exchange for wells near the plate edges. Dimensions: 82.6 by 142.9 mm for standard-size tissue culture plates.

• non-cytotoxic, highly gas permeable
• easily pierceable with pipet tips or pipets for sample recovery
• recommended for temperatures from -20 °C to +80 °C

A 36 µm soft non-permeable aluminum foil sealing film with 28 µm layer of strong medical-grade acrylic adhesive, AlumasSeal IITMsealing films eliminate the need for heat-sealing devices or mats during thermal cycling. Each sealing film measures 82.6 x 146.1 mm and offers sufficient sealing area for all PCR plates. Length between the perforations with end tabs removed is 125.4 mm. Compared to other aluminum foils, AlumaSeal II has less tendency to roll back or itself when removing the backing paper and conforms well to the plate during application.

• easily pierceable with single or multichannel pipettors and robotic probes
• heat & cold resistant, recommended for temperatures from -80 °C to +120 °C
• certified DNase-, RNase-, and nucleic-acid-free
• excellent barrier properties, virtually no sample evaporation or drying

A 38 µm thick aluminum foil sealing film with 38 µm layer of acrylic adhesive for use with 96-well plates. Fits inside the rim of raised-rim plates. AlumaSeal 96TM sealing films have one partial-width end tab with no perforations. Pierceable. Available non-sterile only. Sealing film dimensions are 127.0 mm x 77.8 mm (including single 9.5 mm end tab).

• fits inside rim of raised-rim plate
• heat & cold resistant, recommended for temperatures from -80 °C to +120 °C
• certified DNase-, RNase-, and nucleic-acid-free

A 38 µm thick aluminum foil sealing film with 38 µm layer of acrylic adhesive for use with 384-well plates. AlumaSeal 384TM sealing films have one end tab with no perforations. Pierceable. Available non-sterile only. Sealing film dimensions are 137.3 mm x 82.5 mm (including single 13.5 mm end tab).

• heat & cold resistant, recommended for temperatures from -80 °C to +120 °C
• certified DNase-, RNase-, and nucleic-acid-free

AlumaSeal CSTM sealing films are specially formulated 50 µm aluminum foil sealing films with a 50 µm layer of acrylic adhesive that withstands cold storage at temperatures to -80 °C. Unlike other sealing films in the AlumaSeal group, AlumaSeal CS sealing films are not recommended for PCR or thermocycling. A single non-perforated end tab simplifies application. Simply hold the tab and strip the backing from the body of the sealing film as it lays on the plate to avoid curling. Dimensions are 82.6 by 132.6 mm, including the single 9.5 mm end tab. Recommended temperature range: -80 °C to +120 °C. AlumaSeal CS sealing films are certified DNase-, RNase-, and nucleic-acid-free.

• specially formulated for cold storage to -80 °C
• excellent barrier properties to retard evaporation
• easily pierceable with pipet tips or robotic probes for sample recovery

• Catalog No.	Description	Quantity
  FC-100	AlumaSeal CS Sealing Films, Non-Sterile	100
  FCS-25	AlumaSeal CS Sealing Films, Sterile	50

SealPlate^® ColorTabTM adhesive sealing films consist of 50 µm thickness clear polyester. They minimize evaporation, prevent spillage and contamination between wells, and provide a secure seal for ELISA, EIA, and similar assays. These unique sealing films include a color dot and white writing area on each end tab for plate ID labeling. End tabs with backing removed adhere to the ends of the plate and are perforated so they can be left in place to maintain plate identity even if the center portion of the sealing film is removed. Alternatively, one inscribed color-coded end tab can be removed and pasted in a laboratory notebook while the other remains on the plate for identification. Dimensions 79.4 by 146.1 mm for 96-well plates. Sealing films are supplied with five dot colors (green, lavender, orange, red, blue) in non-sterile single-color packs of 100 and in assorted packs of 50 containing five individual-color packs of 10, also non-sterile.

• color dots and white labeling area on end tabs
• second tab can be separated for lab-notebook insertion
• end tabs can be left on plate even if center of sealing film is removed
• functional temperature range -40°C to +120°C

• Catalog No.	Description	Quantity
  	Non-Sterile:
  SP-IDG-100	SealPlate ColorTab Sealing Films, Green	100
  SP-IDL-100	SealPlate ColorTab Sealing Films, Lavender	100
  SP-IDO-100	SealPlate ColorTab Sealing Films, Orange	100
  SP-IDR-100	SealPlate ColorTab Sealing Films, Red	100
  SP-IDB-100	SealPlate ColorTab Sealing Films, Blue	100
  SP-IDA-10	SealPlate ColorTab Sealing Films, Assorted	50

ThermalSeal RTSTM sealing films are based on 50 µm polyolefin films with 50 µm inert encapsulated silicone adhesive. They are optically clear, with low autofluorescence, and are especially suited for real-time qPCR, storage, and protein crystallization applications. The encapsulated silicone adhesive is non-tacky until pressed against the sealing surface, at which time adhesive is released only in sealing areas to form the strongest available heat-resistant seal around each well on the plate. Adhesive on non-sealing areas of the film, such as directly over sample wells, remains encapsulated and inert. Excel ThermalSeal RTS films are sized to fit within the edges of raised-rim 96-well plates. Their consistent high optical clarity makes possible reproducible, reliable, and consistent DNA amplification measurements and crystal detection. Two end tabs assist in positioning the film on the plate, and the non-tacky adhesive surface simplifies handling. Easy removal of the end tabs at perforated boundaries prevents lifting and higher evaporation rates that can occur with films that overlap the plate rim. Dimensions 78.6 by 139.7 mm. With end tabs removed, length is 113.0 mm.

• high optical clarity
• minimal to no autofluorescence
• chemically inert; no extractables except at extreme pH
• DMSO resistant for HTS
• heat resistant, recommended for temperatures from 
  -70 °C to +100 °C
• certified DNase-, RNase-, and nucleic-acid-free
• fit within raised plate rim to prevent loss of seal due to film lifting
• silicone adhesive forms the strongest available seal for evaporation prevention
• non-tacky adhesive layer simplifies handling of film prior to sealing

• Catalog No.	Description	Quantity
  TSS-RTQ-100	ThermalSeal RTS Sealing Films, Non-Sterile	100

Easily pierceable, 70 µm thick, polyethylene with acrylic adhesive, EZ-PierceTM sealing films allow direct sample recovery with single or multichannel pipettors and robotic probes. Dimensions are 82.6 by 142.9 mm for 96-well plates.

• easily pierceable with pipet tips or robotic probes
• inert, chemical resistant
• functional temperature range -40°C to +90°C

With a 70 µm polyethylene top layer and inert white polypropylene and acrylic adhesive sublayer, this easy-piercing sealing film allows direct sample recovery with single or multichannel pipettors and robotic probes. The adhesive-free zone above each well prevents adhesive fouling of the pipet tip or probe, ultimately minimizing the amount of adhesive that comes into contact with the sample. Dimensions are 79.4 by 142.9 mm for 96-well plates.

• clear zone above each well is free of adhesive and easily pierceable
• clear zones and end tabs facilitate well alignment and accurate positioning
• inert, chemical resistant
• functional temperature range -40 °C to +90 °C

• Catalog No.	Description	Quantity
  ZAF-PE-50	Zone-Free Sealing Films, Non-Sterile	50

Also useful for protecting contents of plate from contamination during multiple manual reagent additions over time. X-PierceTMsealing films are 89 µm thick vinyl with a 18 µm thick adhesive layer, designed for temporary protection from contamination and evaporation for samples in 96-well plates. A precut "X" over each well creates four flaps that easily bend downward when pushed by a robotic probe or pipet tip, allowing sample access without coring or adhesive fouling. A thin adhesive layer and special hairline cuts separating the X-Pierce flaps combine to prevent probes from contacting adhesive and becoming occluded. The flaps return to their original position after sampling for continued protection. For long-term sample protection after sampling, a continuous sealing film should be applied as a second layer. Dimension are 79.4 x 145.5 mm. .

• protect samples and limit evaporation, short-term
• temperatures -40 °C to +90 °C
• special hairline X-cuts and thin adhesive prevent fouling or occlusion of probes and tips
• sealing film reseals for continued sample protection after sampling
•  

• Catalog No.	Description	Quantity
  XP-100	X-Pierce Sealing Films, Non-Sterile	100
  XPS-25	X-Pierce Sealing Films, Sterile	50

SealPlate^® MiniStripsTM sealing films are identical to SealPlate films above, but designed for sealing only one or two 8-well rows at a time on either strip-well or standard plates whenever rows must be selectively protected or accessed. Each MiniStrips film is 19.0 x 102.9 mm, including two positioning tabs. The long dimension with the tabs removed is 82.6 mm. MiniStrips are supplied in sheets of four from which strips can be peeled individually or two-at-a-time for application to plates. Sterile product is packaged in 100 µm tamper-evident bags of 50 sheets of 4 sealing film strips.

• two tabs on each strip for easy application and removal
• perforated tabs can be removed or sealed around plate edge
• can be used as secondary seal to cover sealing films that have been pierced
• 
  • more convenient
  Individual MiniStrips are easlily peeled from sheets of four. The new four-strip-sheet format facilitates extraction from the package, handling, and removal of backing paper before application as compared to the original single-strip configuration.

• Catalog No.	Description	Quantity
  SP-2x8-50	SealPlate MiniStrips Sealing Films, Non-Sterile	200
  SPS-2x8-50	SealPlate MiniStrips Sealing Films, Sterile	200

These improved films for classic thermal cycling applications consist of a 50 µm polyester film with a 41 µm layer of acrylic adhesive. Compared to the original ThermalSeal® films (below), ThermalSeal 2™ films offer better adhesion, reduced evaporation, longer end tabs for easier handling, and less tendency to tear during removal from the plate. Each sealing film measures 79.4 x 139.7 mm and offers sufficient sealing area for all PCR plates. Length between the perforations with end tabs removed is 125.1 mm. (Not recommended for raised-rim plates; see ThermalSeal A™ films below.) ThermalSeal 2 sealing films are not pierceable. For applications where piercing with pipet tips or robotic probes is required for product recovery, see AlumaSeal® sealing films. For real-time PCR applications where maximum optical clarity is required, see ThermalSeal RT and RTS sealing films below.

• heat resistant, recommended for temperatures from -40 °C to +120 °C
• better adhesion; reduced evaporation
• longer end tabs for easier handling
• does not tear during removal from the plate
• easier to apply than aluminum foils, no tendency to roll back
• certified DNase-, RNase-, and nucleic-acid-free

• alog No.	Description	Quantity
  TS2-100	ThermalSeal 2 Sealing Films, Non-Sterile	100
  RL-PLT-01	Accessory: Plate Roller **	1